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Image Search Results
Journal: bioRxiv
Article Title: Potassium channels mediate the inhibitory effect of exosomes on anti-tumor immunity in head and neck cancer
doi: 10.1101/2025.07.25.666838
Figure Lengend Snippet: (A) (Left) Representative KCa3.1 currents recorded in whole-cell voltage-clamp configuration in activated CD8 + T cells from a healthy donor in the absence (Control) or presence of HNSCC exosomes (EXO; 1.04 X 10 9 particles/ ml). (Right) Violin plots of KCa3.1 and Kv1.3 conductance (normalized to cell capacitance) in activated CD8 + T cells from five healthy donors in the absence (Control) or presence of HNSCC exosomes (EXO) from HNC208, HNC285, and HNC365 (n= 21 cells for control and 23 cells for EXO; some donors’ T cells were treated with exosomes from multiple HNSCC patients). Significance was determined using the Mann-Whitney rank sum test. (B) Fold change in mRNA abundance of CALM1 , CALM2, and CALM3 in PBMCs from healthy donors (n=7) treated with HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. The PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and the symbols represent individual treatment conditions. Significance was determined using an unpaired t-test ( CALM2 ) and Mann-Whitney rank sum test ( CALM1 and CALM3 ). (C) Representative flow cytometry staggered histograms showing CaM expression in CD8 + T cells from one healthy donor in the absence (Control) or presence of HNC 208, HNC 285, and HNC 365 exosomes (EXO; 1.04 X 10 9 particles/ ml). The bar graph on the right shows the geometric mean fluorescence intensity (gMFI) for CaM expression in control and EXO-treated cells from four healthy donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Significance was determined by Mann-Whitney rank sum test. (D) Representative Ca 2+ response (shown as a ratio of Indo-1 fluorescence at 400 and 480 nm) recorded in activated healthy donor CD8 + T cells in the absence (Control) or presence of HNC208, HNC285, and HNC365 exosomes (EXO; 1.04 X 10 9 particles/ ml). Cells were loaded with Indo-1; and analyzed by flow cytometry, thapsigargin (arrow) was added in 0 mM Ca 2+ to induce intracellular Ca 2+ store depletion, followed by 2 mM Ca 2+ , which yielded a rapid Ca 2+ influx (see Materials and Methods). Right panel shows the average peak Ca²⁺ fold change in CD8 + T cells from n = 5 donors. Bars represent mean ± SD; symbols represent individual treatment conditions (some donors were treated with exosomes from multiple HNSCC patients). Statistical significance was determined by unpaired t-test. (E) IFN-γ levels in healthy donor CD8 + T cells (n =3 healthy donors) treated with HNSCC exosomes (0.13 × 10 9 particles/ml), isolated from supernatants of HNC208, HNC285, and HNC365 cells, and activated with plate-bound anti-CD3 and anti-CD28 antibodies for 72 h in the absence or presence of 1 μM NS309 (KCa3.1 activator). Each donor was exposed to HNC208, HNC285, and HNC365 exosomes, and the samples were run in duplicate on the same ELISA plate. Activated CD8 + T cells that were not exposed to exosomes were used as controls. For each experiment, IFN-γ levels in control samples were normalized to 1 (dotted line), and all other values were expressed relative to this baseline. Symbols represent individual treatment conditions; bars indicate mean ± SD. Significance was determined using a paired t-test.
Article Snippet: The cells were then resuspended in 100 μl PBS, and stained with Zombie UV Live/Dead dye (BioLegend) as per the manufacturer’s instructions, washed with staining buffer (BioLegend), fixed in 4% paraformaldehyde for 30 minutes and stained overnight at 4°C with guinea pig anti-Kv1.3 and
Techniques: Control, Exosomes, MANN-WHITNEY, Quantitative RT-PCR, Flow Cytometry, Expressing, Fluorescence, Isolation, Enzyme-linked Immunosorbent Assay, FACS, Patch Clamp
Journal: bioRxiv
Article Title: Potassium channels mediate the inhibitory effect of exosomes on anti-tumor immunity in head and neck cancer
doi: 10.1101/2025.07.25.666838
Figure Lengend Snippet: (A) Fold change in mRNA abundance of KCa3.1 (KCNN4 ), Kv1.3 ( KCNA3 ), Orai ( ORAI1 ) and Stim1 ( STIM1 ) in PBMCs from healthy donors (n=7) treated with HNC208, HNC285 and HNC365 exosomes (EXO, 1.04 X 10 9 particles/ ml) was determined by RT-qPCR. PBMCs from some donors in the EXO group were each treated with exosomes from two different HNSCC patients. Each sample was run in triplicate. 18S rRNA was used as the housekeeping gene. Data were normalized to untreated PBMCs (Control). Bars represent means ± SD, and symbols represent individual treatment conditions. Significance was determined by unpaired t-test ( ORAI1 and STIM1 ) and Mann-Whitney rank sum test ( KCNN4 and KCNA3 ). (B-E) Mean fluorescence intensity (MFI) of KCa3.1, Kv1.3, and CD69 in CD3 + (B), CD4 + (C), CD8 + (D), and CD56 dim CD16 + NK cells (E) from four healthy donors treated with HNSCC exosomes from HNC208, HNC285, and HNC365 (same protocol as in A, some donors were treated with exosomes from multiple HNSCC patients). Untreated PBMC were used as controls. Significance was determined by unpaired t-test and Mann-Whitney rank sum test (CD3 + CD69, CD4 + KCa3.1, CD4 + CD69, NK + Kv1.3, NK + CD69). Bars represent means ± SD, symbols represent individual treatment conditions.
Article Snippet: The cells were then resuspended in 100 μl PBS, and stained with Zombie UV Live/Dead dye (BioLegend) as per the manufacturer’s instructions, washed with staining buffer (BioLegend), fixed in 4% paraformaldehyde for 30 minutes and stained overnight at 4°C with guinea pig anti-Kv1.3 and
Techniques: Exosomes, Quantitative RT-PCR, Control, MANN-WHITNEY, Fluorescence
Journal: Membranes
Article Title: Anion- or Cation-Exchange Membranes for NaBH 4 /H 2 O 2 Fuel Cells?
doi: 10.3390/membranes2030478
Figure Lengend Snippet: Polarization curves for DBPFCs employing the two tested membranes, including ( a ) cell voltage and power density curves and ( b ) the corresponding electrodes’ potentials.
Article Snippet: Pt
Techniques:
Journal: Membranes
Article Title: Anion- or Cation-Exchange Membranes for NaBH 4 /H 2 O 2 Fuel Cells?
doi: 10.3390/membranes2030478
Figure Lengend Snippet: Durability tests for DBPFCs employing AMI-7001S and CMI-7000S membrane separators, at zero current flow, with ( a ) the cell voltage decay and ( b ) the corresponding electrodes’ potentials.
Article Snippet: Pt
Techniques: Membrane